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RPA Janssen, kniespecialist voor oa achterste kruisband letsels Standbeencorrectie: informatie, klachten, diagnose en behandeling.
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RPA janssen MD PhD Prof is memeber of the PaTIO study group. Recently, a publication has been...

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Two new publications

Recently 2 new publications have been accepted in peer reviewed journals. These...

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Identifying potential patient-specific predictors for anterior cruciate ligament reconstruction outcome: a diagnostic in vitro tissue remodeling platform

Identifying potential patient-specific predictors for anterior cruciate ligament reconstruction outcome: a diagnostic in vitro tissue remodeling platform

Vijven van M, Groningen van B, Kimenai J, Steen van der MC, Doeselaar van M, Janssen RPA, Ito K, Foolen J
Journal of Experimental Orthopaedics (accepted June 22, 2020)

Abstract

Purpose: Upon anterior cruciate ligament (ACL) rupture, reconstruction is often required, with the hamstring tendon autograft as the most widely used treatment. Post-operative autograft remodeling enhances the risk of graft rupture, which occurs in up to 10% of the patient population, increasing up to 30% of patients aged under 20 years. Therefore, the aim of this research was to identify potential biological predictors for graft rupture, derived from patient-specific tissue remodeling-related cell properties.

Methods: Hamstring tendon-derived cells were obtained from remnant autograft tissue after ACL reconstructions, and seeded in collagen I gels on a micro-tissue platform. Micro-tissue compaction over time – induced by altering the boundary constraints – was monitored. Pro-collagen I expression was assessed using ELISA, and protein expression of tenomodulin and α-smooth muscle actin were measured using Western blot. Expression and activity of matrix metalloproteinase 2 were determined using gelatin zymography. 

Results: Cells were obtained from 36 patients (aged 12-55 years). Only micro-tissues corresponding to younger patients occasionally released themselves from the constraining posts. Pro-collagen I expression was significantly higher in younger patients. Differences in α-smooth muscle actin and tenomodulin expression between patients were found, but these were age-independent. Active matrix metalloproteinase 2 expression was slightly more abundant in younger patients. 

Conclusions: The presented micro-tissue platform exposed patient-specific remodeling-related differences between tendon-derived cells, with the micro-tissues that released from constraining posts and pro-collagen I expression best reflecting the clinical age-dependency of graft rupture. This platform could become a predictive tool to identify individual patients at risk for graft rupture.

Full open access article


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RPA Janssen, MD PhD Prof
Orthopaedic Surgeon
Orthopaedic Associates Eindhoven Greater Area
Orthopedic Center Máxima
Máxima Medical Center (MMC)

Visiting adress MMC Eindhoven:
Ds. Th. Fliednerstraat 1
Ingang Zuid
5631 BM  Eindhoven
The Netherlands 
(outpatient clinic and surgery)

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The Netherlands
(Outpatient clinic)

Mail adress:
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5600 PD  Eindhoven
The Netherlands

Tel. 0031 (0)40 88 88 600
(Call center Orthopedie)

Fax: 0031 (0)40 88 85 938
www.rpajanssen.com 

Links:
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Orthopaedic Associates Eindhoven Greater Area
Máxima Medical Center
Eindhoven University of Technology
Fontys University of Applied Sciences
 

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