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An ex vivo human osteochondral culture model

An ex vivo human osteochondral culture model

Kleuskens MWA, Donkelaar van CC, Kock LM, Janssen RPA, Ito K
J Orthop Res. 2021 Apr;39(4):871-879

Abstract

To reduce animal experimentation and to overcome translational issues in cartilage tissue engineering, there is a need to develop an ex vivo human tissue-based approach. This study aims to demonstrate that a human osteochondral explant at different stages of osteoarthritis (OA) can be kept in long-term culture while preserving its viability and composition. Osteochondral explants with either a smooth or fibrillated cartilage surface, representing different OA stages, were harvested from fresh human tibial plateaus. Explants were cultured for two or four weeks in a double-chamber culture platform. Biochemical content of the cartilage of cultured explants did not significantly change over a period of four weeks and these findings were supported by histology. Chondrocytes mostly preserved their metabolic activity during culture and active bone and marrow were found in the periphery of the explants, while metabolic activity was decreased in the bone core in cultured explants compared to fresh explants. In fibrillated explants, chondrocyte viability decreased in the periphery of the sample in cultured groups compared to fresh explants (fresh: 94±6%, cultured: 64±17%, 2 weeks, and 69±17%, 4 weeks; p < 0.05). Although biochemical and histological results did not show changes within the cartilage tissue, viability of the explants should be carefully controlled for each specific use. This system provides an alternative to explore drug treatment and implant performance under more controlled experimental conditions than possible in vivo, in combination with clinically relevant human osteochondral tissue.

Keywords: articular cartilage, osteochondral, ex vivo, explant, osteoarthritis

 


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RPA Janssen, MD PhD Prof
Orthopaedic Surgeon
Orthopaedic Associates Eindhoven Greater Area
Orthopedic Center Máxima
Máxima Medical Center (MMC)

Visiting adress MMC Eindhoven:
Ds. Th. Fliednerstraat 1
Ingang Zuid
5631 BM  Eindhoven
The Netherlands 
(outpatient clinic and surgery)

Visiting adress MMC Veldhoven:
De Run 4600
5504 DB  Veldhoven
The Netherlands
(Outpatient clinic)

Mail adress:
Postbus 90052
5600 PD  Eindhoven
The Netherlands

Tel. 0031 (0)40 88 88 600
(Call center Orthopedie)

Fax: 0031 (0)40 88 85 938
www.rpajanssen.com 

Links:
KneeGuru
Orthopaedic Associates Eindhoven Greater Area
Máxima Medical Center
Eindhoven University of Technology
Fontys University of Applied Sciences
 

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