Scientific Research

The secret for best clinical practice

slijtage: informatie, klachten, diagnose en behandeling. Knieprothese: Weer een normaal leven! Ervaringen van patiënten op website RPA Janssen, kniespecialist Eindhoven.
Read more →

New publication cooperation Maxima MC & Eindhoven University of Technology

Recently, a scientific paper has...

Read more →

Guest lecture Digital Twin Knee

Prof RPA Janssen MD PhD will present a guest lecture...

Read more →

Guestlecture The Digital twin Knee

Prof RPA Janssen MD PhD will present a guest...

Follow me!

Remain up to date with all the latest knee facts via Facebook, Twitter and other social networks

Evaluating Initial Integration of Cell-Based Chondrogenic Constructs in Human Osteochondral Explants

Evaluating Initial Integration of Cell-Based Chondrogenic Constructs in Human Osteochondral Explants

Meike W.A. Kleuskens, João F. Crispim, Corrinus C. van Donkelaar (Corresponding author), Rob P.A. Janssen, Keita Ito
Tissue Engineering Part C: Methods.Jan 2022.34-44

Integration of an implant with the surrounding tissue is a major challenge in cartilage regeneration. It is usually assessed with in vivo animal studies at the end-stage of implant development. To reduce animal experimentation and at the same time increase screening throughput and speed up implant development, this study examined whether integration of allogeneic cell-based implants with the surrounding native cartilage could be demonstrated in an ex vivo human osteochondral culture model. Chondrocytes were isolated from smooth cartilage tissue of fresh human tibial plateaus and condyles. They were expanded for 12 days either in three-dimensional spinner flask cultures to generate organoids, or in two-dimensional culture flasks for standard cell expansion. Three implant groups were created (fibrin+organoids, fibrin+cells, and fibrin only) and used to fill a Ø 6 mm full-depth chondral defect created in human osteochondral explants (Ø 10 mm, bone length cut to 4 mm) harvested from a second set of fresh human tibial plateaus. Explants were cultured for 1 or 28 days in a double-chamber culture platform. Histology showed that after 28 days the organoids on the interface of the defect remodeled and merged, and cells migrated through the fibrin glue bridging the space between the organoids and between the organoids and the native cartilage. For both conditions, newly formed tissue rich in proteoglycans and collagen type II was present mainly on the edges and in the corners of the defect. In these matrix-rich areas, cells resided in lacunae and the newly formed tissue integrated with the surrounding native cartilage. Biochemical analysis revealed a statistically significant effect of culture time on glycosaminoglycan (GAG) content, and showed a higher hydroxyproline (HYP) content for organoid-filled implants compared with cell-filled implants at both timepoints. This ex vivo human osteochondral culture system provides possibilities for exploration and identification of promising implant strategies based on evaluation of integration and matrix production under more controlled experimental conditions than possible in vivo.

← Back to publications


RPA Janssen, MD PhD Prof
Orthopaedic Surgeon
Orthopaedic Associates Eindhoven Greater Area
Orthopedic Center Máxima
Máxima Medical Center (MMC)

Visiting adress MMC Eindhoven:
Ds. Th. Fliednerstraat 1
Ingang Zuid
5631 BM  Eindhoven
The Netherlands 
(outpatient clinic and surgery)

Visiting adress MMC Veldhoven:
De Run 4600
5504 DB  Veldhoven
The Netherlands
(Outpatient clinic)

Mail adress:
Postbus 90052
5600 PD  Eindhoven
The Netherlands

Tel. 0031 (0)40 88 88 600
(Call center Orthopedie)

Fax: 0031 (0)40 88 85 938

Orthopaedic Associates Eindhoven Greater Area
Máxima Medical Center
Eindhoven University of Technology
Fontys University of Applied Sciences

RPA Janssen YouTube
RPA Janssen Twitter
RPA Janssen Facebook
RPA Janssen LinkedIn
RPA Janssen weblog